Publications


  • Hiroshi Kanno, Kotaro Hiramatsu, Hideharu Mikami, Atsushi Nakayashiki, Shota Yamashita, Arata Nagai, Yin Fei, Keita Tominaga, Omer Faruk Bicer, Ryohei Noma, Bahareh Kiani, Olga Efa, Martin Büscher, Tetsuichi Wazawa, Masahiro Sonoshita, Hirofumi Shintaku, Takeharu Nagai, Sigurd Braun, Jessica P. Houston, Sherif Rashad, Kuniyasu Niizuma, and Keisuke Goda, “High-throughput Fluorescence Lifetime Imaging Flow Cytometry,” Nat Commun. 2024 Sep 4;15:7376. PMC11375057
  • Valentino, K. Ortega-Sandoval, K. D. Houston, and J. P. Houston, "Correlating NAD(P)H lifetime shifts to tamoxifen resistance in breast cancer cells: a metabolic screening study with time-resolved flow cytometry," Journal of Innovative Optical Health Sciences, 2024 https://doi.org/10.1142/S1793545824500202.
  • H. Pouraria and J. P. Houston, "Elasticity of a carrier fluid: A key factor affecting mechanical phenotyping in deformability cytometry," Micromachines (Basel) 2024 Jun 25;15(7):822. Special issue on Application of Microfluidic Technology in Bioengineering, doi: 10.3390/mi15070822 PMC11278870
  • J. P. Houston, S. Valentino, and A. Bitton, "Fluorescence lifetime measurements and analyses: protocols using flow cytometry and high-throughput microscopy," Chapter 15 in Flow Cytometry Protocols, 5th Edition , Teresa S. Hawley and Robert G. Hawley, (Eds.) Methods Molecular Biology, Vol. 2779, 2024, Springer Nature.
  • H. Pouraria, R. Foudazi, and J. P. Houston, "Exploitation of elasto-inertial fluid flow for the separation of nano-sized particles: simulating the isolation of extracellular vesicles," Cytometry A, 2023 Oct;103(10):786-795. doi: 10.1002/cyto.a.24772. Epub 2023 Jun 23; PMC10592338
  • J. Sambrano, F. Rodriguez, J. Martin, and J. P. Houston, "Toward the Development of an On-Chip Acoustic Focusing Fluorescence Lifetime Flow Cytometer," Frontiers in Physics, 2021 9:253 https://www.frontiersin.org/article/10.3389/fphy.2021.647985 
  • A. Bitton, J. Sambrano, S. Valentino, and J. P. Houston, "A Review of New High-Throughput Methods Designed for Fluorescence Lifetime Sensing From Cells and Tissues," Frontiers in Physics, 2021 9:163 https://www.frontiersin.org/article/10.3389/fphy.2021.648553
  • A. Bitton, Y. Zheng, K. D. Houston, and J. P. Houston, "Investigating differences between tamoxifen resistant and sensitive breast cancer cells with flow cytometry," Cytometry A, 2021 99(2):164-169; PMC7986838
  • K. Nichani, J. Li, M. Suzuki, and J. P. Houston, “Evaluation of caspase-3 activity during apoptosis with fluorescence lifetime-based cytometry measurements and phasor analyses,” Cytometry A, 2020 97(12):1265-1275; PMC7738394 
  • F. Alturkistany, K. Nichani, K. D. Houston, and J. P. Houston, “Fluorescence lifetime shifts of NAD(P)H during apoptosis measured by time-resolved flow cytometry,” Cytometry A  2019; 95(1):70-79 PMC6587805 
  • J. Thomas, J. P. Houston, and  L. Boucheron, “Measuring Self-Efficacy in Diverse First-Year Engineering Students Exposed to Entrepreneurial Minded Learning,”  IEEE Frontiers in Education (FIE) 2018 T3G: Engineering Education Research 10.1109/FIE.2018.8658540
  • J. Sambrano, K. Nichani, L. Smagley, A. Chigaev, L. Sklar, J. P. Houston, “Evaluating integrin activation with time-resolved flow cytometry,” Journal of Biomedical Optics 2018; 23(7):1-10 PMCID:PMC6232766
  • J. P. Houston and M. Naivar,  “Methods of measuring fluorescence lifetime using a flow cytometer” United States Patent, Pub no. US9632030 B1, App no. US 14/072,521, Apr 25, 2017.
  • L. Yan, J. Yu, J.P. Houston, N. Flores, and H. Luo, “Biomass derived porous nitrogen doped carbon for electrochemical devices,” Green Energy & Environment,  2017; 2(2):84-99
  • J. Sambrano, Y. Smagley, A. Chigaev, L. A. Sklar, and J. P. Houston, “Using FRET to quantify changes in integrin structures in human leukocytes induced by chemoattractants with multi-frequency flow cytometry,” Proc. SPIE  10062, Optical Interactions with Tissue and Cells XXVIII, 100620X, February 15, 2017.
  • Z. Yang, B. Rutherford, R. McDonald, and J. P. Houston “Development of far-red and ultraviolet digital frequency-domain flow cytometry systems,” IEEE Journal of Selected Topics in Quantum Electronics 2017; 23(3):7100105
  • W. Li, K. D. Houston, and J. P. Houston, “Shifts in the fluorescence lifetime of EGFP during bacterial phagocytosis measured by phase-sensitive flow cytometry,”  Scientific Reports 2017; 7:40341 PMCID:PMC5238435
  • Z. Yang, D. M. Shcherbakova, V. Verkhusha, and J. P. Houston, “Developing a time-resolved flow cytometer for fluorescence lifetime measurements of near-infrared fluorescent proteins,”  Conference on Lasers and Electro-Optics, OSA Technical Digest  2016; https://doi.org/10.1364/CLEO_SI.2016.SW4G.1
  • R. Cao,  Jenkins P, Peria W, Sands B, Naivar M, Brent R, and J. P. Houston, “Phasor plotting with frequency-domain flow cytometry,” Optics Express 2016; 24(13):14596-607  PMCID:PMC5025209
  • E. Dahal, J. Curtiss, D. Subedi, G. Chen, J. P. Houston, and S. Smirnov, “Evaluation of the Catalytic Activity and Cytotoxicity of Palladium Nanocubes,” ACS Appl. Mater. Interfaces 2015; 7 (18): 9364–9371  PMCID:PMC4663053
  • P. Jenkins, M. Naivar, and J. P. Houston, “Toward the measurement of multiple fluorescence lifetimes in flow cytometry: maximizing multi-harmonic content from cells and microspheres,” Journal of Biophotonics 2015; 8(11-12):908-917  PMCID:PMC4869968
  • B. Sands, P. Jenkins, B. J. Peria, M. Naivar, J. P. Houston, and R. Brent, “Measuring and sorting cell populations expressing isospectral fluorescent proteins with different fluorescence lifetimes,” PLoS One 2014; 9(10):e109940  PMCID:PMC4193854
  • R. Cao, M. Naivar, M. Wilder, and J. P. Houston, “Expanding the potential of standard flow cytometry by extracting fluorescence lifetimes from cytometric pulse shifts.,” Cytometry A  2014; 85(12):999-1010 PMCID:PMC4257068  **selected for the 2014 Best Paper Award
  • W. Li, G. Vacca, and J. P. Houston, “Fluorescence lifetime excitation cytometry by kinetic dithering,” Electrophoresis 2014; 35(12-13):1846-54 PMCID:PMC4231566
  • A. Gohar, R. Cao, P. Jenkins, W. Li, J.P. Houston, and K. D. Houston, “Subcellular localization-dependent changes in EGFP fluorescence lifetime measured by time-resolved flow cytometry,” Biomedical Optics Express 2013; 4(8):1390-1400  PMCID:PMC3756581
  • R. Cao, V. Pankayatselvan, and J. P. Houston, “Cytometric sorting based on the fluorescence lifetime of spectrally overlapping signals,” Optics Express 2013; 21(12): 14816-14831 PMCID:PMC3726248
  • J. P. Houston, M. Naivar, P. Jenkins and J. P. Freyer, “Capture of fluorescence decay times by flow cytometry,” Current Protocols in Cytometry 2012;  59(1):1.25.1-1.25.12  PMCID:PMC4240630
  • J. P. Houston, M. Naivar, and J. P. Freyer, “Digital Acquisition of fluorescence lifetime by frequency domain flow cytometry,” Cytometry A  2010; 77A(9):861- 872  PMCID:PMC2930036 
  • E. M. Sevick-Muraca, R. Sharma, J. C. Rasmussen, M. V. Marshall, J. A.Wendt, H. Q. Pham, E. Bonefas, J. P. Houston, L. Sampath, K. E. Adams, D. Blanchard, R.E. Fisher, S. Chiang, R. Elledge, and M. E. Mawad, “Imaging of lymph flow in breast cancer patients after microdose administration of a near-infrared fluorophore – feasibility,” Radiology 2008, 246(3) 734-741.
  • R. Sharma, W. Wang, J. Rasmussen, A. Joshi, J. P. Houston, K. Adams, A. Cameron, S. Ke, M. Mawad, and E. Sevick, “Quantitative imaging of lymph function,” American Journal of Physiology: Heart and Circulation 2007, 292:H3109-H3118.
  • J. P. Houston, S. Ke, W. Wang, C. Li, and E. M. Sevick-Muraca, “Quality analysis of in vivo NIR fluorescence and conventional gamma images acquired using a dual-labeled tumor-targeting probe,” Journal of Biomedical Optics 2005, 10(5):54010.
  • K. Hwang, J. P. Houston, J. Rasmussen, S. Ke, C. Li, and E. M. Sevick-Muraca, “Enhanced fluorescent optical imaging with improved excitation light rejection,” Molecular Imaging 2005, 4(3):194-204.
  • S. Kwon, S. Ke, J. P. Houston, W. Wang, Q. Wu, C. Li, and E. M. Sevick-Muraca, “Imaging dose-dependent pharmacokinetics of an RGD-fluorescent dye conjugate targeted to avb3 receptor expressed in Kaposi’s sarcoma,” Molecular Imaging 2005, 4:75-87 (cover of issue).
  • C.Li, W. Wang, Q. Wu, S. Ke, J. P. Houston, E. M. Sevick-Muraca, L. Dong,  D. Chow, C. Charnsangavej and J. P. Gelovani, “Dual optical and nuclear imaging in human melanoma xenografts using a single targeted imaging probe,” Nuclear Medicine and Biology 2006, 33(3):349-358.
  • J. P. Houston and E. M. Sevick-Muraca, “Sensitivity and depth penetration of CW versus FDPM NIR fluorescence contrast-enhanced imaging,” Photochemistry and Photobiology 2003, 77:420-430.